5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
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At that time, the early indicators have been dismissed as debris, however the retrospective analysis demonstrated shared qualities While using the problematic cells in society. Tracing the infection helped the lab set up added laboratory strategies for screening and protection of assay-significant cell strains.
Whether or not you’re a starter or an authority, check out our means to progress your research and abilities in flow cytometry.
Mix acoustic focusing engineering with large-parameter Evaluation for versatile spectral unmixing and conventional workflows.
Find out new insights into your experimental information by visualizing populations working with mixed fluorescence and morphology data. Our application interface wizards empower downsampling, concatenation (merged files) and dimensionality applications which have been created in the application for an intuitive, streamlined info analysis practical experience.
"An image just isn't pretty really worth one thousand dots, nonetheless it seriously helps you to see photos. It is great to determine illustrations or photos that correlate with staining. One example is, AnnexinV-positive cells which might be morphologically different than healthier cells, or CD14+ monocytes that are much larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a qualified consumer has gated singlets confidently. Immediately after analyzing the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate is made up of a lot more than four% aggregates.
Pair your Attune flow cytometer having an autosampler for elevated workflow performance, and insert robotic integration for maximum throughput.
Consistent graphic quality even at substantial throughput Acoustic concentrating plus a higher-speed digital camera Blend to impression these T cells continuously at low or superior flow premiums. Easily change aim and digicam settings to satisfy experimental specifications.
Early indications of this contamination had initially been dismissed as particles. Backgating to find new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also means that you can use morphological capabilities to find fascinating subpopulations that could not be apparent from flow cytometry data on your own.
Consumers can perspective function illustrations or photos in genuine-time to verify sample top quality, optimize protocols, and file the morphology of every mobile population recognized for Evaluation. Imaging seize also Positive aspects from acoustic concentrating, which helps you to posture the cells making sure that a pointy, centered impression is obtained.
AI-pushed automatic image Assessment While using the Attune Cytometric Software program translates event characteristics into distinctive morphology parameters which might be combined with standard fluorescence and scatter parameters to reveal extremely reproducible populace modifications and exceptional subsets.
We Beforehand shown the power of imaging Auto-T/Ramos cell interactions. Enable’s examine just the population of biggest curiosity, the double good gatherings, to learn more. We could now use prolonged morphological parameters (below, circularity and skewness of depth) to additional look at the options of these populations and refine gating on these activities, raising information robustness.
Constructed-in dimensionality reduction instruments increased resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to confirm predicted populace resolution in lysed entire human blood. User can view occasions from Every cluster for Visible affirmation of mobile phenotypes.
No wash/no lyse leukocyte Investigation with imaging. Cells were acquired from 24-hour-outdated blood by dilution in 1 mM EDTA ( Pro UX/UI permits users to practice AI styles for expanded applications. (A) The “Prepare Model” element gives annotation applications and true-time product instruction precision.
Gating on these extended parameters means check here that you can swiftly and properly recognize populations of fascination and confirm gating method with little if any guide review. The Attune program presents entry to point out-of-the-artwork machine Discovering Assessment instruments in just an expertly intended consumer interface, making it extremely simple to use.
As the cell line had Beforehand been analyzed on the Attune CytPix Flow Cytometer, the researchers went back again to the photographs and were being capable of document the microbial infection not less than five times previously.
Agilent Price Assure guarantees you not less than 10 years of instrument use from the day you buy. If not, We are going to credit rating you While using the residual value of the method toward an upgraded design.
What gave the impression to be a homogeneous inhabitants is really a lot more numerous—an insight that ought to be regarded when interpreting success.